Research Paper Volume 11, Issue 17 pp 6872—6891

Cholesterol lowering attenuates pressure overload-induced heart failure in mice with mild hypercholesterolemia

Figure 7. Cholesterol lowering gene therapy in TAC mice reduces pro-oxidative enzymes, increases anti-oxidant defence systems, and decreases nitro-oxidative stress in the myocardium. Bar graphs illustrating plasma TBARS expressed as plasma malondialdehyde equivalents (A), plasma xanthine oxidase activity (B), myocardial protein level of NADPH oxidase 2 (C), of NADPH oxidase 4 (D), and of superoxide dismutase (E), and plasma superoxide dismutase activity (F) (n=8 for each condition). Percentage of 3-nitrotyrosine-positive area in the myocardium in control sham (n=14), AAV8-LDLr sham (n=11), control TAC (n=25), and AAV8-LDLr TAC (n=11) mice 8 weeks after operation (G). Data are expressed as means ± SEM. Representative images of western blots are shown in panel h. Representative photomicrographs showing myocardial sections stained for 3-nitrotyrosine (I). Scale bar represents 100 μm. Insets show a 4x magnification of the boxed region.