Research Paper Volume 11, Issue 18 pp 7386—7401

lncRNA DLEU2 modulates cell proliferation and invasion of non-small cell lung cancer by regulating miR-30c-5p/SOX9 axis

Figure 2. lncRNA DLEU2 knockdown inhibited cell proliferation, invasion, migration, and induced apoptosis. (AB) Cell viability in transferred with si-DLEU2 and pcDNA-DLEU2 were determined by CCK-8 assay; #p<0.05, ##p<0.01, compared with the pcDNA-NC group; (C) colony formation of A549 and LLC cells was determined; (D) The number of invasion cell was detected by Transwell assay; (E) The migration abilities of tumor cells were assessed by wound healing assay; (F) The percentage of apoptosis cell was measured by flow cytometry; (GH) The expression of apoptosis-related proteins Bax and Caspase-3 were inhibited in si-DLEU2 group by western blotting, while promoted Bcl-2 expression in pcDNA-DLEU2 group compared with NC group. *p<0.05, **p<0.01, compared with NC group.