Research Paper Volume 11, Issue 18 pp 7386—7401

Figure 4. SOX9 was the target of miR-30c-5p. (A) The bioinformatics analysis result showed that miR-30c-5p had a binding site with SOX9; (B) Dual-luciferase reporter gene assay was used to confirm the target relationship between miR-30c-5p and SOX9; (C–D) The expression of SOX9 mRNA was detected by qRT-PCR and western blotting in A549 and LLC cells which were transferred with miR-30c-5p; (E) qRT-PCR was used to determine the expression of SOX9 in NSCLC tissues and adjacent tissues; (F) The expression relationship between miR-30c-5p and SOX9 was evaluated by Spearman’s correlation analysis; (G–H) Western blotting was applied to detect the expression of SOX9 protein in A549 and LLC cells. ^*p<0.05, ^**p<0.01, compared with NC group; ^##p<0.01, compared with adjacent tissues.