Research Paper Volume 11, Issue 17 pp 6983—6998

Curcumin suppresses osteogenesis by inducing miR-126a-3p and subsequently suppressing the WNT/LRP6 pathway

Figure 1. The effect of different concentrations of curcumin on osteogenic differentiation of hADSCs. (A) qRT-PCR detected the mRNA levels of osteogenic-related genes in curcumin- treated cells on day 6. (B) Western blot assays analysed the protein levels osteogenic related genes in curcumin-treated cells. (C, D) ALP staining and ALP activity analyses indicated early differentiation on day 6. (E) Alizarin red staining was performed to detect calcium salt deposits on day 12. Scale bars: 200 μm. Quantitative data are presented as the mean ± S.D. (n =3). *P<0.05; **P<0.01; ***P<0.001. Representative images are shown.