Figure 4. The suppressive effect of curcumin on osteogenic differentiation is antagonized in miR-126a-3p-inhibited hADSCs. (A) hADSCs were transfected with the miR-126a inhibitor (anta-126a) or the negative control (anta-NC) and were treated with curcumin or untreated. The mRNA levels of osteogenic-related genes were detected by qRT-PCR assay on day 6 of osteogenesis. (B) The protein levels of osteogenic-related genes were detected by western blot assay in osteogenic-induced cells. (C, D) ALP staining, and ALP activity analyses were performed to indicate the early differentiation on day 6 of osteogenic differentiation. (E) Alizarin red staining was performed to detect calcium salt deposits on day 12. Scale bars: 200 μm. Quantitative data are presented as the mean ± S.D. (n =3). *P<0.05; **P<0.01; ***P<0.001.