Research Paper Volume 11, Issue 17 pp 6983—6998

Curcumin suppresses osteogenesis by inducing miR-126a-3p and subsequently suppressing the WNT/LRP6 pathway

Figure 5. Prediction and verification of miR-126a-3p target genes. (A) Bioinformatic analysis was used to predict the binding seed sequence of miR-126a-3p with the 3′UTR of LRP6. The wild type (WT) or mutant (MUT) 3′UTR fragments of LRP6 were inserted into the psiCHECK-2 reporter vector. (B) The relative luciferase activities were detected using a Dual-Luciferase Reporter Assay System. (C, D) The mRNA and protein levels of LRP6 were analyzed by qRT-PCR and western blot assays.