Research Paper Volume 11, Issue 17 pp 6999—7020

Prognostic value and immune infiltration of novel signatures in clear cell renal cell carcinoma microenvironment

Figure 2. Differential expressed genes with immune and stromal score and related functional annotations in ccRCC. (A) Based on immune score comparison, 162 genes were up-regulated and 747 genes down-regulated in the high score than the low score group after propensity analysis using limma package algorithm. (B) Similarly, for high stromal score compared with low score, 261 up-regulated genes and 1198 down-regulated genes were obtained. (CD) A total of 77 DEGs were commonly upregulated in the high scores groups, and 787 genes were synchronously downregulated using Venn algorithm. (E) functional enrichment analysis including GO: BP, GO: CC, GO: MF and KEGG pathways, was performed in 864 commonly DEGs. (F) Cluster analysis and heat map including 77 up-regulated DEGs suggested distinct mRNA expression profiles of DEGs in 533 ccRCC samples.