Figure 2. Differential expressed genes with immune and stromal score and related functional annotations in ccRCC. (A) Based on immune score comparison, 162 genes were up-regulated and 747 genes down-regulated in the high score than the low score group after propensity analysis using limma package algorithm. (B) Similarly, for high stromal score compared with low score, 261 up-regulated genes and 1198 down-regulated genes were obtained. (C–D) A total of 77 DEGs were commonly upregulated in the high scores groups, and 787 genes were synchronously downregulated using Venn algorithm. (E) functional enrichment analysis including GO: BP, GO: CC, GO: MF and KEGG pathways, was performed in 864 commonly DEGs. (F) Cluster analysis and heat map including 77 up-regulated DEGs suggested distinct mRNA expression profiles of DEGs in 533 ccRCC samples.