Research Paper Volume 11, Issue 17 pp 7021—7035

Figure 1. The migration, proliferation, secretion and angiogenesis of EPCs declined with aging. (A, B) Scratch test of EPCs isolated from young, middle-aged and aged mice, at 0 and 24 h. Magnification, ×100. (C, D) Representative images of crystal violet-stained migrated cells, as assessed by a Transwell assay. Magnification, ×400. The number of migrated EPCs was calculated. (E, F) The proliferative ability of EPCs was assessed based on the percentage of EdU-positive cells (red). Scale bar: 100 μm. (G, H) Representative images displaying tube-forming activity. Magnification, ×100. (I) The secretion of VEGF and NO into the supernatants of cultured EPCs. *P<0.05. n=5. Y, young; MA, middle-aged; A, aged.