Figure 1. The migration, proliferation, secretion and angiogenesis of EPCs declined with aging. (A, B) Scratch test of EPCs isolated from young, middle-aged and aged mice, at 0 and 24 h. Magnification, ×100. (C, D) Representative images of crystal violet-stained migrated cells, as assessed by a Transwell assay. Magnification, ×400. The number of migrated EPCs was calculated. (E, F) The proliferative ability of EPCs was assessed based on the percentage of EdU-positive cells (red). Scale bar: 100 μm. (G, H) Representative images displaying tube-forming activity. Magnification, ×100. (I) The secretion of VEGF and NO into the supernatants of cultured EPCs. *P<0.05. n=5. Y, young; MA, middle-aged; A, aged.