Figure 2. NRF2 expression in cultured EPCs was reduced with advancing age. Low-magnification images of NRF2 expression in different age groups are shown in (A), with quantification in (B). High-magnification confocal images of NRF2 expression are shown in (C). NRF2 expression was detected in the cytoplasm (white arrows) and/or nuclei (red arrows) in younger cells, while little or no NRF2 expression was detected in middle-aged and aged cells (yellow arrows). *P<0.05. n=5. Y, young; MA, middle-aged; A, aged. Scale Bar: (A) 100 μm, (C) 20 μm.