**Figure 1.** **HCC specific SA-LncRNAs was downregulated during cellular senescence, and ***miat* downregulation promoted cellular senescence. (**A**) Schematic overview of the study design. (**B**, **C**) Volcano plot of differentially expressed genes in proliferating vs. senescent WI-38 cells and HCC vs. normal tissues, respectively. The x-axis indicates log_{2} fold changes between the two groups and the y-axis indicates the -log_{10} adjusted p-value of gene expression variation. The upregulated genes are shown as red dots, the downregulated genes are shown as blue dots and the normal genes are shown as grey dots. (**D**) Real-time PCR analysis for *miat* expression in 2BS cells. The bars represent the mean and SD of three independent experiments, *P < 0.05, **P< 0.01, *** P< 0.001. (**E**) Cellular senescence assay by *SA-β-gal* staining in 2BS cells induced by the oncogene *ras.* (**F**) Cell cycle distribution analysis measured by propidium iodide staining and flow cytometry in 2BS cells induced by the oncogene *ras.* (**G**) Real-time PCR analysis for *miat* expression in 2BS cells induced by the oncogene *ras.* The bars represent the mean and SD of three independent experiments, *P < 0.05, **P< 0.01, *** P< 0.001. (**H**, **L**, **P**) Real-time PCR analysis for *miat* expression in 2BS cells, IMR-90 and MRC-5 cells transfected with the sh-*miat* plasmid. The bars represent the mean and SD of three independent experiments, *P < 0.05, **P< 0.01, *** P< 0.001. (**I**, **M**, **Q**) Cellular senescence assay by *SA-β-gal* staining in 2BS, IMR-90 and MRC-5 cells. (**J**, **N**, **R**) Cell cycle distribution analysis measured by propidium iodide staining and flow cytometry in 2BS, IMR-90 and MRC-5 cells (n=3). (**K**, **O**, **S**) Cell proliferation analysis determined by CCK assay (n=4, mean ± SD) in 2BS, IMR-90 and MRC-5 sh-*miat* cells (n =3, mean ± SD; absorption at 450 nm was detected at 0, 24, 48, 72 h and 96 h after transfection). *P < 0.05, **P< 0.01, *** P< 0.001.