Research Paper Volume 11, Issue 18 pp 7416—7441

Hearing consequences in Gjb2 knock-in mice: implications for human p.V37I mutation

Figure 3. Endocochlear potential (EP) measurement and Stria Vascularis morphology in KI mice. (A) Significantly reduced EP in senile homozygous mice (ranging from 60 weeks old to 90 weeks old) (Left panel, ***P <0.0001, t=5.876, df=16.6, Student’s unpaired t-test with Welch’s correction). Symbols represent individual measurement and lines are the means. EP reduction of homozygous mice remained stable within the expanded timeline from 3 postnatal weeks to ~2 years (Right panel). Linear regressions showed that slopes of both groups were virtually zero (fitted functions are shown in the right panel, both P>0.05, linear regression). (B) Representative H&E staining of Stria vascularis for all three turns with cross-sectional areas outlined for quantitative analysis (scale bar, 40 μm). (C) Histogram illustrating the averaged cross-sectional area of Stria Vascularis of 60 weeks old cochleae (Mean + SEM). The cross-sectional area showed no significant difference between wild-type and homozygous mice (P>0.05, two-way ANOVA followed by Bonferroni post-test).