Figure 6. Spiral ganglion neuron (SGN) and inner hair cell (IHC) synapse count in KI mice at different ages. (A) Representative H&E staining images of SGNs at 20 and 60 week-old homozygous and wild-type mice (Scale Bar, 20 μm). (B) SGN density (Number of SGNs/area of Rosenthal’s canal) showing no significant difference between wild-type and homozygous mice at both time points (P>0.05 at both time points, two-way ANOVA followed by Bonferroni post-test). (C) Representative confocal images of IHC synapses. Dotted line outlines a single IHC (Scale Bar, 5 μm). (D) The numbers of CTBP2 puncta per IHC at both 20 and 60 weeks old showing no significant difference between two genotype groups (P>0.05 at both age groups, two-way ANOVA followed by Bonferroni post-test).