Research Paper Volume 11, Issue 18 pp 7416—7441

Hearing consequences in Gjb2 knock-in mice: implications for human p.V37I mutation

Figure 7. Inner hair cell (IHC) patch clamp recordings in KI mice. (A) Representative calcium currents (ICa) induced by voltage ramps in IHCs of wild-type and homozygous mice. (B) The ICa peak of homozygous mice was significantly lower than wild-type mice (**P= 0.0091, t=2.978, df=15.49, Student’s unpaired t-test with Welch’s correction). (C) Vhalf, and (D) slope of Ica showed no significant difference between the two genotype groups (P>0.05, Student’s unpaired t-test with Welch’s correction). (E) Representative whole-cell ICa and membrane capacitance (Cm) traces. Exocytosis was triggered by calcium current in response to a single step depolarization. (F) Homozygous mice exhibited no significant membrane capacitance change (ΔCm), (G) calcium charge (Q) or (H) ΔCm/Q, representing Ca2+ efficiency in triggering exocytosis (all P>0.05, two-way ANOVA followed by Bonferroni post-test).