Research Paper Volume 11, Issue 18 pp 7416—7441

Hearing consequences in Gjb2 knock-in mice: implications for human p.V37I mutation

Figure 8. ABR analysis and EP measurement in KI mice after noise exposure. (A) ABR audiograms tracking changes of thresholds after one episode of a two-hour, 100 dB SPL, 8-16 kHz band-pass noise exposure. ABR thresholds of both groups recovered to the baseline level at 15 days after noise exposure (P>0.05 for both genotypes, two-way ANOVA followed by Bonferroni post-test). (B) EPs didn’t change in both wild-type and homozygous mice at day 15 of noise exposure (P>0.05, Student’s unpaired t-test with Welch’s correction). Baseline EPs were obtained by pooling data from young individuals less than 20 weeks old in Figure 3. (C) Comparison of Wave I latencies of day 0 (pre-exposure) and day 15 at 90dB SPL showing that homozygous mice presented a tendency of prolonged latency in frequencies above 11.3 kHz (*P=0.021, F(1,5)=11.003 at 16 kHz and *P=0.016, F(1,5)=12.961 at 22.6 kHz, two-way ANOVA followed by Bonferroni post-test). (D) Summary of ABR Wave I amplitude showing no significant difference between the two genotypes (P>0.05 for all frequencies, two-way ANOVA followed by Bonferroni post-test). ΔAmplitude=day 15 amplitude at 90 dB SPL - day 0 amplitude at 90 dB SPL.