Figure 9. IHC synapse and OHC count in KI mice after noise exposure. (A) Representative confocal images of IHC CTBP2 puncta before and after noise exposure. (Scale Bar, 5μm). (B) Histograms summarizing averaged number of CTBP2 puncta at different turns (Mean + SEM). Reduction of CTBP2 was only observed at the basal turn in homozygous mice 15 days after noise exposure (*P=0.024, F(1,4)=12.423, two-way ANOVA followed by Bonferroni post-test). (C) Representative confocal images of hair cells before and after noise exposure. (Scale Bar, 20μm). (D) OHC survivals at day 0 and 15 were compared. No significant hair cell loss was observed in both genotypes in all three turns (all P>0.05, two-way ANOVA followed by Bonferroni post-test). Baseline images and data were obtained from the same group of 20-week-old animals as in Figure 6.