Figure 5. Downregulation of miR-221 attenuated proliferation and fibrosis-related proteins. (A) The efficiency of inhibitor-miR-221 was measured by qPCR; (B) CCK8 assay results showed that miR-221 inhibition suppressed MC proliferation; (C–E) mRNA expression level of FN (C), Col-4 (D), and TGFβ1 (E) in MCs transfected with inhibitor-miR-221; (F, G) The protein expression level of FN, Col-4, and TGF-β1 were measured by western blot. MCs were transfected with inhibitor-miR-221. *P < 0.05, **P < 0.01.