Research Paper Volume 11, Issue 20 pp 8745—8759

Long noncoding RNA GAS5 inhibits cell proliferation and fibrosis in diabetic nephropathy by sponging miR-221 and modulating SIRT1 expression

Figure 7. lncRNA GAS5 upregulated SIRT1 expression and decreased the proliferation and fibrosis-related proteins by acting as miR-221 sponge. (AF) qPCR and western blot assays revealed that GAS5 upregulation increased SIRT1 expression and GAS5 downregulation decreased SIRT1 expression; (GI) qPCR and western blot results showed that the expression level of SIRT1 in the MCs. The MCs were transfected with LV-GAS5 or mimic-miR-221; (JL) expression level of Col-4 in MCs was measured by qPCR and western blot. The MCs were transfected with LV-GAS5 or mimic-miR-221; (MO) p53 expression level in MCs was measured by qPCR and western blot. MCs were transfected with LV-GAS5 or mimic-miR-221. *P < 0.05, **P < 0.01.