Research Paper Volume 11, Issue 20 pp 8760—8776

Adipose tissue-derived omentin-1 attenuates arterial calcification via AMPK/Akt signaling pathway

Figure 1. Effects of omentin-1 on osteoblastic differentiation and mineralisation of calcifying vascular smooth muscle cells (CVSMCs). (A) Effects of omentin-1 on the ALP activity in CVSMCs. The cells were cultured with or without omentin-1 (100-400 ng/mL) for the indicated time points. The ALP activity was measured by an ALP kit, normalized to the cellular protein contents. *p < 0.05, one-way ANOVA with the Tukey’s HSD post hoc analysis. (B) Effects of omentin-1 on osteocalcin (OC) secretion in CVSMCs. The cells were treated with or without omentin-1 (100–400 ng/mL) for 48 hours, and then OC secretion of the cells was determined by radioimmunoassay, normalized to the cellular protein contents. *p < 0.05, one-way ANOVA with the Tukey’s HSD post hoc analysis. (C) Effects of omentin-1 on Runx2 expression in CVSMCs. The cells were cultured for 48 hours with or without 100-400 ng/mL omentin-1. The data were presented as densitometric ratios of Runx2/β –actin. *p < 0.05, one-way ANOVA with the Tukey’s HSD post hoc analysis. (D) Effects of omentin-1 on calcium deposition. The cells were cultured for 14 days with or without omentin-1. *p < 0.05, unpaired Student’s t-test. (E) Alizarin Red S staining view of either vehicle or omentin-1 treated CVSMCs for 14 days. Representative microscopic pictures were shown. All Results are represented by mean ± SD with 3 replicates for each group.