Research Paper Volume 11, Issue 18 pp 7473—7491

HnRNPR-CCNB1/CENPF axis contributes to gastric cancer proliferation and metastasis

Figure 1. hnRNPR was overexpressed in gastric cancer cell lines and patients. (A) The bioinformatics analysis revealed that hnRNPR was upregulated in stomach tissues compared to normal tissues. (B) The GEPIA dataset indicated that the level of hnRNPR in cancer tissues was higher compared to that in normal tissues. T(Tumors)=408; N(Normal)=211. (C) Three GEO database (GSE13861, GSE27342, GSE13911 from GEO database) revealed that the expression of hnRNPR was significantly higher in tumors compared with normal tissues. (D) The relative mRNA level of gastric cell lines (SGC-7901, AGS, HGC-27, MKN-28, MGC-803) were elevated than in normal epithelial cell line GES-1. GAPDH served as the internal control. (E) The protein level of hnRNPR in gastric cell lines were higher than in normal cell lines GES-1. GAPDH served as the internal control. Each experiment was performed in triplicate and repeated three times. P values were calculated with two-tailed unpaired Student’s t test. *, P<0.05, **, P<0.01, ***, P<0.001, ****, P<0.0001 versus the control.