Research Paper Volume 11, Issue 18 pp 7473—7491

HnRNPR-CCNB1/CENPF axis contributes to gastric cancer proliferation and metastasis

Figure 4. HnRNPR promoted cancer aggressiveness by binding CENPF mRNA. (A) GSEA analysis indicated that high hnRNPR expression positively correlated with three metastasis signatures. (B) Venn graph indicated that CENPA, CENPN, RFC4, BUB1, BIRC5, AURKA, CENPF, DLGAP5, ECT2, and CCNB2 were overlapped in the three groups. (C) The expression of CENPF was positively correlated with that of hnRNPR. (D) The CENPF expression in gastric tumors was significantly higher than that in normal tissues in TAGC-STAD database. (E) RIP-PCR revealed that CENPF RNA is enriched in the hnRNPR group compared to the control. (F) Two (HGC-27 and SGC-7901) cell lines control and hnRNPR cells were transfected with shRNA control or shRNA against CENPF for Immuno-blotting. (G) Migration and (H) invasion assays showed that inhibition of CENPF rescued the aggressiveness induced by hnRNPR in SGC7901 and HGC-27 cell lines. Data were from three independent experiments performed in triplicate. P values were calculated with two-tailed unpaired Student’s t test. The expression correlation was determined with Pearson’s correlation analysis. *, P<0.05, **, P<0.01.