Research Paper Volume 11, Issue 17 pp 7242—7256

Reactive oxygen species-mediated senescence is accelerated by inhibiting Cdk2 in Idh2-deficient conditions

Figure 1. Idh2 was downregulated in senescent mouse embryonic fibroblasts (MEFs) and aging tissues. (A) SA-ß-gal staining of Passage 2 (P2) and Passage 8 (P8) MEFs. Scale bar, 200 µm. (B) Statistical analysis of SA-ß-gal-stained positive cells between P2 and P8 MEFs. (C) Western blot analysis between P2 and P8 MEFs. The following antibodies were used: anti-p53, anti-p16, anti-p21, and anti-ß-actin. (D) Detection of Idh2 expression level in P2 and P8 MEFs. Western blotting and reverse-transcriptase PCR was performed for detecting Idh2. (E)Relative protein expression of Idh2 was detected in mouse tissues using western blot analysis. Ten-week-old and 47-week-old mice were used. Data are expressed as means ± SD (n = 3). *p < 0.05, **p < 0.01, and ***p < 0.001.