Figure 4. Effects of miR-27a on the Akt activation and GLUT expression in IR 3T3-L1 cells. Cells were transfected with antagomiR-27a or antagomiR-NC, and then treated with TNF-α (10 ng/ml) and/or insulin (100 nM) in high-glucose DMEM containing FBS (10%, w/v). (A) WB displayed that antagomiR-27a transfection led to GLUT upregulation and PPAR-γ expression and increased Akt phosphorylation, while showing no effect on Akt levels in IR 3T3-L1 cells. (B) Image pixel analysis of phosphorylated Akt band. (C) qPCR assay showed GLUT mRNA expression in IR 3T3-L1 cells transfected with antagomiR-27a. (D) IFA showed that GLUT4 staining was increased after antagomiR-27a transfection. Number = 3. *P < 0.05, compared to indicated groups.