Research Paper Volume 11, Issue 18 pp 7510—7524

MiR-27a promotes insulin resistance and mediates glucose metabolism by targeting PPAR-γ-mediated PI3K/AKT signaling

Figure 5. miR-27a downregulation promote insulin sensitive via targeting PPAR-γ. (A) Glucose level in IR 3T3-L1 cells that underwent different treatment was examined to assess the effects of PPAR-γ inhibitor T0070907 (1 μM) on insulin sensitivity. (B) WB was used to detect the PPAR-γ, Akt, GLUT expression, and Akt phosphorylation in cells with or without T0070907 treatment (1 μM) for 24 h. (C) qPCR determined that the PPAR-γ expression was inhibited by T0070907. (D) Image pixel analysis showed phosphorylated level Akt protein after T0070907 treatment. (E) qPCR was performed to confirm that T0070907 treatment downregulated the glucose level, which was induced by agomiR-27a transfection. Number = 3. **P < 0.01, compared to indicated groups.