Research Paper Volume 11, Issue 20 pp 8777—8791

LncRNA MALAT1 shuttled by bone marrow-derived mesenchymal stem cells-secreted exosomes alleviates osteoporosis through mediating microRNA-34c/SATB2 axis

Figure 4. BMSCs-derived exosomal MALAT1 promotes osteoblast activity. (A) The MALAT1 expression in exosomes evaluated by RT-qPCR. (B) The MALAT1 and miR-34c expression in hFOB1.19 detected by RT-qPCR. (C) The protein expression of SATB2, Runx2, ATF4 and Hoxa2 in osteoblasts (hFOB1.19) treated with oe-MALAT1 detected by Western blot analysis. (C) Alizarin red staining of osteoblasts (hFOB1.19) treated with oe-MALAT1 (200 ×). L. ALP staining of osteoblasts (hFOB1.19) treated with oe-MALAT1 (200 ×). * p < 0.05 vs. oe-NC. Data were expressed with mean ± standard error. In Panel AC, the unpaired t test was used for data analysis. The experiment was repeated three times.