Research Paper Volume 11, Issue 20 pp 8777—8791

LncRNA MALAT1 shuttled by bone marrow-derived mesenchymal stem cells-secreted exosomes alleviates osteoporosis through mediating microRNA-34c/SATB2 axis

Figure 6. Upregulated MALAT1 alleviates the symptoms of osteoporosis in mice. OVX mice were injected with oe-MALAT1 + miR-34c agomir or miR-34c antagomir + sh-SATB2. (A) The MALAT1 and miR-34c expression as well as the mRNA expression of SATB2 in femoral tissue was detected by RT-qPCR. (B) The protein expression of SATB2 in femoral tissue by Western blot analysis. (C) Three-dimensional CT image of distal femur (1 mm). (DH) Quantitative data of BV/TV (D), Tb.N (E), Tb.Th (F), BMD (G) and Tb.Sp (H) in distal femoral of mice. (I) Number of osteoblasts in vertebral body sections. (J) Calcitonin double labeling analysis. * p < 0.05 vs. oe-NC + agomir-NC; # p < 0.05 vs. oe-MALAT1 + agomir-NC; & p < 0.05 vs. miR-34c antagomir + sh-NC. Data were expressed with mean ± standard deviation. In Panel BF, the unpaired t test was used for data analysis.