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Research Paper Volume 11, Issue 20 pp 8792—8809
Long non-coding RNA MALAT1 promotes cardiac remodeling in hypertensive rats by inhibiting the transcription of MyoD
Figure 6. Over-expression of lncRNA MALAT1 promotes cell proliferation and fibrosis, which, whereas, is reversed by up-regulating MyoD. (A) the expression of lncRNA MALAT1 in ASMCs of SHRs, detected by RT-qPCR; (B–C) the expression of MyoD in ASMCs of SHRs, detected by Western blot analysis; (D) ASMC activity in SHRs, evaluated using MTT assay; (E) proliferation of ASMCs in SHRs, determined by BrdU assay (× 200); (F) cell cycle analysis in SHRs, determined by flow cytometry; (G) the expression of Bax and Bcl-2 in ASMCs of SHRs detected using Western blot analysis; (H) the expression of fibronectin and collagen I in ASMCs, tested by Western blot analysis; *, p < 0.05 vs. the LV-CON-vector group; #, p < 0.05 vs. the LV-MALAT1 + LV-CON-vector group; measurement data were expressed by means ± standard deviation; data in panel (A–E, G and H) were analyzed by one-way analysis of variance; data in panel (F) were analyzed by repeated-measures analysis of variance; n = 3.