Research Paper Volume 11, Issue 17 pp 7294—7306

Impact of NF-κB pathway on the apoptosis-inflammation-autophagy crosstalk in human degenerative nucleus pulposus cells

Figure 2. Effects of NF-κB inhibition and LPS on human NPCs. (A) The morphology of the primary NP cells was observed with inverted microscope (magnification ×200). (B) The viability of NPCs induced by various concentrations of LPS was measured by CCK-8. (C) Western blot analysis for the protein expression of P65. (D) The nuclear translocation of P65 was measured by Fluorescence immunocytochemistry (magnification ×200). Values are means ±SEM.*p<0.05 vs. control group, #p<0.05 vs. LPS group.