Research Paper Volume 11, Issue 17 pp 7294—7306

Impact of NF-κB pathway on the apoptosis-inflammation-autophagy crosstalk in human degenerative nucleus pulposus cells

Figure 5. Inhibiting NF-κB blocks apoptosis and inflammatory of degenerative human NP cells by promoting autophagy. (A)After NPCs were pre-treated by autophagy inhibitor CQ, western blot analysis for the expression of LC3 II, cleaved caspase3, TNF-α and IL-1β. (B) The apoptosis rate was detected by flow cytometry. (C) Real-time analysis for the expression of TNF-α and IL-1β. Values are means ±SEM.*p<0.05 vs. control group, #p<0.05 vs. P65-siRNA+LPS group.