Research Paper Volume 11, Issue 18 pp 7553—7569

Long non-coding RNA TUG1 enhances chemosensitivity in non-small cell lung cancer by impairing microRNA-221-dependent PTEN inhibition

Figure 3. Overexpression of TUG1 enhanced the apoptosis, autophagy and senescence of NSCLC cells in response to DDP. (A) the effect of TUG1 on the cell cycle distribution of NSCLC cells; (B) the effect of TUG1 on apoptosis of NSCLC cells; (C) GFP-LC fusion protein tagging detection of autophagosome formation; (D) MDC staining to detect formation of autophagic vacuoles; (E) expression of autophagy-related proteins and cell senescence-related protein hTERT determined by Western blot analysis, normalized to β-actin. ** p < 0.01 versus the control group.