Figure 1. Pathway analysis of the unfolded protein response mRNA transcripts at baseline. Baseline UPR genes transcripts are not different at baseline in young vs. older adults (A) The x-axis represents the log fold change, while y-axis represents the –log FDR-value for each gene. UPR genes are colored in blue. Dotted vertical lines represent the threshold for statistical significance (FDR < 0.05, −log [FDR value] > 1.303). Gene set enrichment analysis (GSEA) of 72 gene transcripts associated with the UPR reveals a trend towards upregulation of mRNA for the unfolded protein response in skeletal muscle under basal conditions (B) in the older compared to younger adults (FDR < 0.05 and an absolute log2 fold change R ≥ 0.5).