Research Paper Volume 11, Issue 18 pp 7639—7662

miR-125a-5p inhibits tumorigenesis in hepatocellular carcinoma

Figure 6. miR-125a-5p suppresses cell proliferation and induces apoptosis in HCC by targeting PTPN1 and MAP3K11 via the MAPK signaling pathway. (AD) Cell proliferation ability was assessed using CCK8 and colony formation assays after Bel-7404 and SK-Hep1 cells were transfected with miR-control, miR-125a-5p inhibitors, PTPN1 siRNA 3, or MAP3K11 siRNA 1. Two-way ANOVA, mean ± SD, **P<0.01, ***P<0.001. (E and F) Immunofluorescence staining of cleaved caspase substrate was detected after transfection in Bel-7404 and SK-Hep1 cells. Scale bars: 100μm. (G and H) Expression of PTPN1, MAP3K11, JNK1/2/3, and c-Jun was examined in transfected Bel-7404 and SK-Hep1 cells using Western blots.