Research Paper Volume 11, Issue 18 pp 7723—7745

Neural stem cell small extracellular vesicle-based delivery of 14-3-3t reduces apoptosis and neuroinflammation following traumatic spinal cord injury by enhancing autophagy by targeting Beclin-1

Figure 3. Overexpression of 14-3-3t enhances the effect of NSC-sEVs on the repair of functional behaviors following spinal cord injury. (A) BBB scores at different time points following spinal cord contusion. (B) Bevel test at different time points after spinal cord contusion. (C) Representative footprints of animal-walking 28 days after SCI. Blue: paw print on the front paw; red: paw print on the hind paw. (D) Gross morphology. (E) Representative H&E-stained sagittal section. Scale bar = 1000um. (F) Quantitative analysis of lesion volume between each group. * p < 0.05, compared to the SCI group; # p < 0.05, compared to the Ad-GFP-sEVs group. NSC-sEVs, neural stem cell-derived small extracellular vesicles; SCI, spinal cord; BBB, Basso, Beattie, and Bresnahan.