Research Paper Volume 11, Issue 18 pp 7723—7745

Neural stem cell small extracellular vesicle-based delivery of 14-3-3t reduces apoptosis and neuroinflammation following traumatic spinal cord injury by enhancing autophagy by targeting Beclin-1

Figure 7. The effect of 14-3-3t overexpression or knockdown in NSC-sEVs on lipopolysaccharide-induced secretion of pro-inflammatory cytokines in microglia cells. (AC) Following the overexpression of 14-3-3t in NSC-sEVs, ELISA was used to detect the release of the pro-inflammatory cytokines, TNF-a, IL-1β and IL-6 in microglia supernatants. (DF) After 14-3-3t was knocked-out in NSC-sEVs, ELISA was used to detect the release of the pro-inflammatory cytokines, TNF-a, IL-1β and IL-6 in microglia supernatant. * p < 0.05, compared to the LPS group, # p < 0.05, compared to the Ad-GFP-sEVs or shGFP-sEVs groups. NSC-sEVs, neural stem cell-derived small extracellular vesicles; TNF-a, tumor necrosis factor alpha; IL-1β, interleukin-1β; IL-6, interleukin-6; LPS, lipopolysaccharide.