Research Paper Volume 11, Issue 18 pp 7746—7779

Inadequate control of thyroid hormones sensitizes to hepatocarcinogenesis and unhealthy aging

Figure 2. The modulation of THs alters glucose homeostasis in adulthood. (A) Circulating glucose levels during an OGTT. n = 8 per group. Repeated measurements two-way ANOVA. See also Supplementary Figure 2A. (B) AUC for the OGTT. Two-way ANOVA. (C) Circulating insulin levels during an OGTT. n = 8 per group. Repeated measurements two-way ANOVA. See also Supplementary Figure 2B. (D) AUC for the insulin levels in the OGTT. Two-way ANOVA. (E) Circulating glucose levels during an IPPTT. n = 8 per group. Repeated measurements two-way ANOVA. See also Supplementary Figure 2C. (F) AUC for the IPPTT. Two-way ANOVA. (G) Circulating glucose levels during an ITT. n = 8 per group. Repeated measurements two-way ANOVA. See also Supplementary Figure 2D. (H) AUC for the ITT. Two-way ANOVA. (I) Western blots showing total Akt and pSer473 Akt in liver extracts of Wt and Pax8 +/- mice. Mice were 9 month-old at time of death and were fasted 16 hours prior killing. (J) Densitometric analysis of western blots shown in panel I. n = 6 per group. T-test two tailed. (K) Western blots showing total Akt and pSer473 Akt in gastrocnemius extracts of Wt and Pax8 +/- mice. Mice were 9 month-old at time of death and were fasted 16 hours prior killing. (L) Densitometric analysis of western blots shown in panel K. n = 6 per group. T-test two tailed. (M) Glucose levels in blood after 16 hours of fasting. n = 8 per group. Two-way ANOVA. (N) Circulating insulin levels after 16 hours of fasting. n = 8 per group. Two-way ANOVA. (O) HOMA-IR. n= 8 per group. Two-way ANOVA. (P) Percentage of HbA1c. n = 7 per group. Two-way ANOVA. Data are represented as the mean ± SEM. Unless otherwise stated, mice were 7-month old at the time of experimentation. h: hours. a.u.: arbitrary units. * p-value < 0.05 between Wt mice and Pax8 +/- mice. & p-value < 0.05 between Wt mice and Wt T4 mice. # p-value < 0.05 between Wt mice and Pax8 +/- T4 mice.