**Figure 3.** **High fat diet (HFD) feeding does not exacerbates the metabolic phenotype of Pax8 +/- mice.** (**A**) T4 levels in serum (18 weeks of HFD feeding). n = 8 Wt, n = 9 Pax8 +/-. T-test two tailed. (**B**) Body weights during HFD feeding. n = 15 Wt, n = 14 Pax8 +/-. Repeated measurements two-way ANOVA. (**C**) Basal daily energy intake at 6 months of life (13 weeks of HFD feeding). n = 8 Wt, n = 9 Pax8 +/-. T-test two tailed. (**D**) Fasting induced energy intake (17 weeks of HFD feeding). n = 8 Wt, n = 9 Pax8 +/-. T-test two tailed. (**E**) Circulating glucose levels during an OGTT at 6 months of life (14 weeks of HFD feeding). n = 13 Wt, n = 11 Pax8 +/-. Repeated measurements two-way ANOVA. (**F**) AUC for glucose levels during the OGTT. T-test two tailed. (**G**) Insulin levels in plasma during an OGTT (17 weeks of HFD feeding). n = 8 per group. Repeated measurements two-way ANOVA. (**H**) AUC for insulin levels during the OGTT. T-test two tailed. (**I**) Circulating glucose levels during the IPPTT (15 weeks of HFD feeding). n = 13 Wt, n = 10 Pax8 +/-. Repeated measurements two-way ANOVA. (**J**) AUC for the IPPTT. T-test two tailed. (**K**) Glucose levels in blood during the ITT (16 weeks of HFD feeding). n = 9 per group. Repeated measurements two-way ANOVA. (**L**) AUC for the ITT. T-test two tailed. (**M**) Glucose levels in blood after 16 hours of fasting (18 weeks of HFD feeding). n = 8 per group. T-test two tailed. (**N**) Circulating insulin after 16 hours of fasting (18 weeks of HFD feeding). n = 8 per group. T-test two tailed. (**O**) HOMA-IR (18 weeks of HFD feeding). n = 8 per group. T-test two tailed. Data are represented as the mean ± SEM. * p-value <0.05. Unless otherwise stated, mice were 7-month old at the time of experimentation.