Research Paper Volume 11, Issue 18 pp 7746—7779

Inadequate control of thyroid hormones sensitizes to hepatocarcinogenesis and unhealthy aging

Figure 3. High fat diet (HFD) feeding does not exacerbates the metabolic phenotype of Pax8 +/- mice. (A) T4 levels in serum (18 weeks of HFD feeding). n = 8 Wt, n = 9 Pax8 +/-. T-test two tailed. (B) Body weights during HFD feeding. n = 15 Wt, n = 14 Pax8 +/-. Repeated measurements two-way ANOVA. (C) Basal daily energy intake at 6 months of life (13 weeks of HFD feeding). n = 8 Wt, n = 9 Pax8 +/-. T-test two tailed. (D) Fasting induced energy intake (17 weeks of HFD feeding). n = 8 Wt, n = 9 Pax8 +/-. T-test two tailed. (E) Circulating glucose levels during an OGTT at 6 months of life (14 weeks of HFD feeding). n = 13 Wt, n = 11 Pax8 +/-. Repeated measurements two-way ANOVA. (F) AUC for glucose levels during the OGTT. T-test two tailed. (G) Insulin levels in plasma during an OGTT (17 weeks of HFD feeding). n = 8 per group. Repeated measurements two-way ANOVA. (H) AUC for insulin levels during the OGTT. T-test two tailed. (I) Circulating glucose levels during the IPPTT (15 weeks of HFD feeding). n = 13 Wt, n = 10 Pax8 +/-. Repeated measurements two-way ANOVA. (J) AUC for the IPPTT. T-test two tailed. (K) Glucose levels in blood during the ITT (16 weeks of HFD feeding). n = 9 per group. Repeated measurements two-way ANOVA. (L) AUC for the ITT. T-test two tailed. (M) Glucose levels in blood after 16 hours of fasting (18 weeks of HFD feeding). n = 8 per group. T-test two tailed. (N) Circulating insulin after 16 hours of fasting (18 weeks of HFD feeding). n = 8 per group. T-test two tailed. (O) HOMA-IR (18 weeks of HFD feeding). n = 8 per group. T-test two tailed. Data are represented as the mean ± SEM. * p-value <0.05. Unless otherwise stated, mice were 7-month old at the time of experimentation.