Research Paper Volume 11, Issue 18 pp 7746—7779

Inadequate control of thyroid hormones sensitizes to hepatocarcinogenesis and unhealthy aging

Figure 4. Mild hypothyroid Pax8 +/- mice exhibit reduced basal metabolic rate and compromised physical performance. (A) Basal daily energy intake per day at 10-12 months of life. n = 5 Wt, n = 4 Pax8 +/- (the n reflects the number of cages). (B) Fasting-induced energy intake at 10-12 months of life. n = 21 Wt, n = 14 Pax8 +/-. (C) Lipid content in faeces. n = 7 Wt, n = 6 Pax8 +/-. (D) Body temperature. n = 12 Wt, n = 8 Pax8 +/-. (E) Determination of RER in metabolic cages. n = 8 Wt, n = 7 Pax8 +/-. (F) Determination of heat production in metabolic cages. n = 8 Wt, n = 7 Pax8 +/-. (G) Determination of spontaneous locomotor activity (y + x axis) in metabolic cages. n = 8 Wt, n = 7 Pax8 +/-. (F) Determination of run distance in metabolic cages. n = 8 Wt, n = 7 Pax8 +/-. (H) Triglyceride concentration in plasma. n = 18 Wt, n = 13 Pax8 +/-. (I) Total cholesterol, HDL and LDL/VLDL levels in serum. n = 7 per group. (J) Blood levels of Gpt. n = 7 per group. (K) Blood levels of Got. n = 7 per group. (L) Time to fall from an accelerating rotarod at 7-10 months of life. n = 27 Wt, n = 20 Pax8 +/-. (M) Time to fall in wire hang test at 7-10 months of life. n = 27 Wt, n = 20 Pax8 +/-. Data are represented as the mean ± SEM. Unless otherwise stated, mice were 7-month old at the time of experimentation. * p-value < 0.05. T-test two tailed.