Research Paper Volume 11, Issue 18 pp 7780—7795

Sulfated polysaccharide of Sepiella maindroni ink targets Akt and overcomes resistance to the FGFR inhibitor AZD4547 in bladder cancer

Figure 6. Combination of SIP-SII and AZD4547 enhances growth suppression of RT112 xenografts. Nude mice were randomly divided into four experimental groups after subcutaneous injection of RT112 cells. (A) Tumor volume measurements. (B) Tumor weight measurements after euthanasia on day 28 post-treatment initiation. (C) Body weight measurements. ***P < 0.001 vs. DMSO control; n = 3 per group. (D) Immunohistochemical staining of phospho-Akt in excised tumors (vehicle, SIP-SII, AZD4547, and combination therapy). Representative images at 100x magnification. (E) Western blot analysis of tumor samples.