Figure 4. Effects of compound 33 on the MAPK/NF-κB and Nrf2/HO-1 signaling pathways in vivo. (A) p-P38, (B) p-ERK, (C) p-JNK, (D) p-IκBα, (E) P65, and (F) p-P65 protein levels relative to total P38, total ERK, total JNK, and GAPDH (loading control) were assayed using their respective antibodies. (G) Translocation of NF-κB P65 visualized by immunofluorescence staining. Immunohistochemical analysis of (H) Nrf2 and (I) HO-1 expression in lung tissue. Data are presented as means ± SEM (n = 3–6). # P <0.05, ## P <0.01, compared to the control group. * P <0.05, ** P <0.01, compared to the LPS group.