Figure 3. Effects of PRL-3-silencingon cell proliferation and apoptosis were evaluated in K562 and K562/G01 cells. (A) A cell growth curve was plotted based on the OD value (proportional to cell numbers) obtained at different time points following transfection. (B, C) Colonies containing ≥40 cells were counted on day 7 using a microscope (×200). (D) Cells were labeled by PI and analyzed using FCM. (E) Apoptotic cells were measured by FCM. Dot plots show 7-AAD (y-axis) vs. Annexin-V (x-axis). (*P<0.05, **P<0.01, vs. NC group).