Figure 4. Effects of PRL-3-silencing on cell migration and invasion were evaluated in K562 and K562/G01 cells. (A) The OD values (proportional to cell numbers) of migrated cells were measured by MTS assay. (B) The invasion cell numbers were counted under microscope in five HP fields. (C) Wright-Giemsa stained invasion cells were observed under microscope (×200). (D, E) Quantification of MMP2 and MMP9 were normalized to β-actin. (F) MMP2, MMP9, PRL-3 and β-actin expression by western blot. (*P<0.05, **P<0.01, vs. NC group).