Research Paper Volume 11, Issue 18 pp 7830—7846

Downregulation of LncRNA-XIST inhibited development of non-small cell lung cancer by activating miR-335/SOD2/ROS signal pathway mediated pyroptotic cell death

Figure 3. The effects of LncRNA-XIST on NSCLC cell apoptosis. (A) FCM was used to detect A549 cell apoptosis and (B) quantification was conducted. (C) The expression levels of apoptosis associated proteins (Caspase-3, Bax and Bcl-2) in A549 cells, which were normalized to β-actin and (D) quantified by Image J software. (E) FCM was used to detect H1299 cell apoptosis and (F) quantification was conducted. (G) Western Blot was used to detect Caspase-3 levels in H1299 cells, which were normalized to β-actin and (H) quantified by Image J software. (I, J) The apoptosis ratio of HBE cells was detected by FCM. (“NS” represented no statistical significance, “*” represented p < 0.05, “**” represented p < 0.01).