Research Paper Volume 11, Issue 20 pp 8825—8844

ACADS acts as a potential methylation biomarker associated with the proliferation and metastasis of hepatocellular carcinomas

Figure 4. ACADS promotes apoptosis and it suppresses tumorigenicity in HCC. (A) With the help of the Hochest 3342 and the Mito Traker staining assays, it can be observed that apoptosis was reduced by the knockdown of ACADS in Huh7 and HCCLM3 cells. (B and C) The Tumor xenograft model. When Tumor xenografts were designed and used, it was noted that the tumors in the shACADS group showed significantly faster growth rates as compared to those tumors which belonged to the shNC group. The typical tumor section samples were isolated and analyzed. (D and E) A Statistical analysis was conducted which indicated that there was a significant reduction in tumor size and weight. (* P<0.05, **P<0.01).