Figure 6. The CpG Island methylation levels of ACADS are regulated by DNMTs in HCC cells. (A) According to the results obtained with the help of the Starbase database, the expression of ACADS is negatively correlated with that of DNMT1, DNMT3A, and DNMT3B. (B–G) The expression of ACADS in HCCLM3 and Huh7 cells was significantly increased after treatment with 5-Aza. It can also be seen that the mRNA and protein expression of ACADS were both increased if the DMNT genes were silenced. (* P<0.05, **P<0.01).