Research Paper Volume 11, Issue 18 pp 7880—7898

Mechanisms of ROS-induced mitochondria-dependent apoptosis underlying liquid storage of goat spermatozoa

Figure 3. Effects of liquid storage on the mitochondrial membrane potential (MMP) and expression changes in mitochondria-dependent apoptosis proteins in goat spermatozoa. JC-1 staining with a fluorescence microscope (A) and flow cytometric analysis (B) showed change in the MMP. (C) Western blot analysis of Cleaved caspase-9, Cleaved caspase-3, and Cytochrome c (CytC) protein expressions in goat spermatozoa. *P < 0.05 compared to 0 h.