Figure 5. Validation of ROS-induced mitochondria-dependent apoptosis during the liquid storage of goat spermatozoa by the addition of the ROS scavenger NAC (0 mM, 5 mM, and 10 mM) at 96 h of storage. (A) Change in the intracellular ROS levels. (B) Assessment of spermatozoa motility. (C) Evaluation of the apoptosis rate by TUNEL staining. (D) Change in the MMP by JC-1 staining. (E) Protein expression of Cleaved caspase-9, Cleaved caspase-3, and Cytochrome c (CytC) by western blot analysis. *P < 0.05 compared to 0 mM.