Research Paper Volume 11, Issue 18 pp 7914—7937

TIP-B1 promotes kidney clear cell carcinoma growth and metastasis via EGFR/AKT signaling

Figure 3. TIP-B1 promotes proliferation, migration and invasion of KIRC cells. (A) mRNA level of TIP-B1 in different KIRC cell lines and normal HK2 cell line. (B) protein level of TIP-B1 in different KIRC cell lines and normal HK2 cell line. (CD) Efficiencies of TIP-B1 knockdown in OS-RC-2 cells (C) and 786-O cells (D) were validated by RT-PCR (left) and western blot(right) assays. (EF) Cell proliferation was analyzed by CCK8 assay in OS-RC-2 cells (E) and 786-O cells (F). (GH) Wound-healing assay after TIP-B1 knockdown in OSRC-2 (G) and 786-O (H) cells when compared to that of pLKO control cells. (IJ). Transwell migration (I) and invasion (J) assay after TIP-B1 knockdown in OSRC-2 (I) and 786-O (J) cells when compared to that of control cells.