Research Paper Volume 11, Issue 18 pp 7978—7995

GSK-3β and ERK1/2 incongruously act in tau hyperphosphorylation in SPS-induced PTSD rats

Figure 2. SPS-induced PTSD leads to tau hyperphosphorylation at specific sites, but doesn’t alter Aβ1-40 and/or Aβ1-42 production. (A) Hippocampal protein levels of total tau, p-tau at Ser199, Ser202/Thr205(AT8), Ser396 and Ser404 were measured by WB. (B and C) Statistical analysis showed a marked increased at AT8(Ser202/Thr205) and Ser404 in SPS rats. (D and E) Statistical analysis of the level of pS199 and pS396 showed no significant differences between the two groups. (F) Total tau (tau5) normalized by DM1A. (G) Immunohistochemistry showed the increased distribution of AT8 compared to control (upper row scale bar=500μm, middle and lower row scale bar=100μm). (H and I) Measurement of Aβ1-40 and Aβ1-42 by ELISA kits in hippocampus. All data represent mean ± SEM, n=3, *P < 0.05, **P < 0.01 vs control, ****P<0.0001 vs control.