Research Paper Volume 11, Issue 18 pp 7978—7995

GSK-3β and ERK1/2 incongruously act in tau hyperphosphorylation in SPS-induced PTSD rats

Figure 3. SPS modulates tau-related kinases not phosphatase. (A) The level of total and phosphorylated GSK3β at Ser9 and Tyr216 were measured by WB. (B and C) SPS rats showed a decreased phosphorylation of GSK3β at Ser9 (B) and a significant increase at Tyr216 (C). (D) Total GSK3β didn’t change compared with control. (E, F and G) WB showed no changes in the levels of both PP2Ac and phosphor-PP2Ac-Y307. (H, I and J) CaMKII and p- CaMKII were detected by Western blot and showed no significant difference. (K, L and M) The level of p-ERK1/2 was significantly decreased in SPS rats and total ERK1/2 showed no changes compared with control. All data represent mean ± SEM, n=3, *P < 0.05, **P < 0.01 vs control, ****P<0.0001 vs control.