Research Paper Volume 11, Issue 18 pp 7978—7995

GSK-3β and ERK1/2 incongruously act in tau hyperphosphorylation in SPS-induced PTSD rats

Figure 5. SGK1 overexpression upregulates the p-ERK1/2 and increases tau hyperphosphorylation at Ser199 and Ser396 in HEK293/tau cells. (A) HEK293/tau cells were transfected with SGK1 plasmid, and WB was performed to detect p-ERK1/2, ERK1/2 and SGK1. (B) Quantification showed the significantly increased level of SGK1. (C and D) SGK1 overexpression increased phosphorylation of ERK1/2. (E) Overexpression of SGK1 evidently increased the level of pS199 and pS396 compared with control. (FH) Quantitative analysis for blot in E. (I) Model showing how downregulated SGK1 and AKT contribute to PTSD. All data represent mean ± SEM, n=3, *P < 0.05 vs control, **P < 0.01 vs control.