Research Paper Volume 11, Issue 20 pp 8860—8878

Long non-coding SBF2-AS1 acting as a competing endogenous RNA to sponge microRNA-142-3p to participate in gemcitabine resistance in pancreatic cancer via upregulating TWF1

Figure 4. Effect of knock-down of SBF2-AS1 expression on epithelial-mesenchymal transition, invasion and migration of drug-resistant pancreatic cancer cells. (A) The morphology of cells is observed by an inverted microscope (× 100). (B) The mRNA expression of transcriptional factors Twist and ZEB1 in EMT detected by RT-qPCR. (C) The expression of E-cadherin and Vimentin detected by western blot analysis. (D) The invasion ability of AsPC-1/GEM and PANC-1/GEM cells determined by Transwell assay (× 400). (E) The migration ability of AsPC-1/GEM and PANC-1/GEM cells determined by Transwell assay (× 400). Repetitions = 3; Data was analyzed using one-way ANOVA.